Generator

Part:BBa_K620003:Design

Designed by: Caltech iGEM 2011   Group: iGEM11_Caltech   (2011-09-20)


Tet promoter + BisdB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 545
    Illegal BamHI site found at 933
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 70
    Illegal AgeI site found at 1342
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1152


Design Notes

A repeat sequence in the promoter made PCR assembly and Gibson assembly extremely difficult. Ultramers were ordered to prevent mispriming.


Source

Assembled from BBa_R0040, BBa_K123001, BBa_B0014.

References